Measurement of barnase refolding rate constants under denaturing conditions
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چکیده
منابع مشابه
Comparative Production Analysis of Three Phlebovirus Nucleoproteins under Denaturing or Non-Denaturing Conditions for Crystallographic Studies
Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production under both non-denaturing and denaturing/refolding conditions can be investigated and compared. T...
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Danson, M. J. & Perham, R. N. (1976) Biochem. J. 159, 677-682 Danson, M. J., Hooper, E. A. & Perham, R. N. (1978a) Biochem. J. 175, 193-198 Danson, M. J., Fersht, A. R. & Perham, R. N. (1978b) Proc. Natl. Acad. Sci. U.S.A. in the press Durchschlag, H. (1975) Biophys. Struct. Mech. 1 169-188 Eley, M. H., Namihara, G., Hamilton, L., Munk, P. & Reed, L. J. (1972) Arch. Biochem. Biophys. 152, 655-6...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1994
ISSN: 0014-5793
DOI: 10.1016/0014-5793(94)00384-x